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KMID : 0613820040140020291
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Journal of Life Science
2004 Volume.14 No. 2 p.291 ~ p.295
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Neuronal Protection by Rooibos (Aspalathus linearis) Tea Infusions in a Hypoxic Model of Cultured Rat Cortical Neurons
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Moon Il-Soo
Ko Bok-Hyun
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Abstract
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Rooibos (Aspalathus linearis) (RB) is a leguminous shrub native to the mountainous areas of the northwestern Cape Province in South Africa. RB tea infusions are the fermentation products of its leaves and fine stems, and known to have a high antioxidative activity due to the presence of flavonoids and phenolic acids. We investigated the effects of RB tea on the alleviation of oxidative stress on cultured rat cortical neurons in a hypoxic model. Measurement of lactate dehydrogenase (LDH) released into culture media revealed that RB increased cell viabilities in both normoxia (6- 18%) and hypoxia (2-24%) dose-dependently (10-100 μg/ml) on 16 days in vitro (3 days after treatment). Visualization of cell morphology by expression of GFP-Hsc70 fusion protein showed that RB (50 μg/ml) reduced the average vacuolated soma from 55.4±4.59% (no RB addition) to 40.9±6.3% (RB addition) on 5 days after hypoxia. Our results proves efficacy of RB in the neuroprotection of hypoxic neurons and extend application for RB into the prevention and/or treatment of neuronal damages.
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KEYWORD
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cortical culture, hypoxia, LDH, neuron, Rooibos
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